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Rumen microbial variation and nutrient utilisation in mithun ( Bos frontalis ) under different feeding regimes
Author(s) -
Prakash B.,
Saha S. K.,
Khate K.,
Agarwal N.,
Katole S.,
Haque N.,
Rajkhowa C.
Publication year - 2013
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/j.1439-0396.2011.01270.x
Subject(s) - rumen , fibrobacter succinogenes , propionate , neutral detergent fiber , zoology , randomized block design , dry matter , fermentation , population , digestion (alchemy) , amylase , biology , straw , isovalerate , cellulase , food science , chemistry , butyrate , agronomy , biochemistry , hydrolysis , enzyme , demography , chromatography , sociology
Summary The aim of the study was to investigate the effect of feeding different diets on fermentation, enzyme activities and microbial population in the rumen fluid of mithun ( Bos frontalis ). In a randomized block design, 20 male mithun (6–8 months of age, 152 ± 12.6 kg body weight) were randomly divided into four experimental groups ( n = 5/group) and fed experimental diets ad libitum for 180 days. The diet R 1 contained tree foliages (TF), R 2 comprised of 50% concentrate mixture (CM) and 50% TF, R 3 contained 50% CM and 50% rice straw, and R 4 contained 50% CM, 25% TF and 25% rice straw. Rumen liquor was collected at 0 and 180 days of the experiment for estimation of different ruminal parameters and a digestion trial was conducted at the end of the experiment. Rumen fluid was analysed for pH, ammonia nitrogen (NH 3 ‐N), total‐N, ruminal enzymes, short chain fatty acid (SCFA) and microbial profile. The relative quantification of ruminal microbes was carried out with real‐time PCR using bacteria as the house keeping gene. The dry matter intake, nutrients digestibility, body weight gain, NH 3 ‐N, total‐N, carboxymethyl cellulase, avicelase, xylanase, amylase, protease and molar proportion of butyrate were (p < 0.05) higher in mithun fed R 2 , R 3 and R 4 compared to those fed R 1 diet. In contrast, increased (p < 0.05) ruminal pH, molar proportion of acetate and, acetate to propionate ratio was recorded in mithun fed only TF than those fed concentrate supplemented diets. Similarly, an increase (p < 0.05) in the population of Fibrobacter succinogenes , Ruminococcus flavefaciens and total bacteria were evident in mithun fed R 2 , R 3 and R 4 compared to those fed R 1 . Therefore, it is concluded that TF 25% and/or rice straw 25% along with CM 50% may be fed to the growing mithun for improved rumen ecology, nutrient utilization and thus better performance under stall fed system.