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Inhibition of ruminal microbial methane production by β‐cyclodextrin iodopropane, malate and their combination in vitro
Author(s) -
Mohammed N.,
Lila Z. A.,
Ajisaka N.,
Hara K.,
Mikuni K.,
Hara K.,
Kanda S.,
Itabashi H.
Publication year - 2004
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/j.1439-0396.2004.00456.x
Subject(s) - propionate , chemistry , ammonia , fermentation , food science , zoology , molar , biochemistry , nuclear chemistry , biology , paleontology
Summary The objective of this study was to evaluate the effects of different concentrations of l ‐malate (0, 5, 10 and 20 m m ), 2‐iodopropane‐ β ‐cyclodextrin complex (CD‐IP) (0, 0.1, 0.2 and 0.4 m m ) and a combination of malate (10 and 20 m m ) plus CD‐IP (0.2 and 0.4 m m ) on methane production from corn starch. Ruminal fluid was collected from dairy cows, mixed with phosphate buffer (1 : 2) and incubated (30 ml) anaerobically at 38 °C for 6 h with or without additives. Fermentation of corn starch in the presence of malate resulted in an increase (p < 0.05) in pH of the medium, total volatile fatty acid (VFA), total gas production and molar proportion of propionate. Acetate and ammonia‐N concentration were unchanged. Methane production was decreased (p < 0.05) (15.5 to 20.4%). Addition of CD‐IP in corn starch resulted in an increase (p < 0.05) in total VFA and molar proportion of propionate. Acetate, pH and ammonia‐N concentration of the medium were decreased (p < 0.05). Total gas production was unchanged. Methane production was decreased (p < 0.05) (25.2 to 97.1%) and hydrogen production was increased (p < 0.05). Addition of l ‐malate to CD‐IP resulted in an increase (p < 0.05) in total VFA, total gas production and molar proportion of propionate. Acetate and ammonia‐N concentration were decreased (p < 0.05). No effects were observed on medium pH. Methane production was decreased (p < 0.05) (49.5 to 97.1%). Hydrogen production was also decreased (p < 0.05) (54.5 to 64.1%) compared with those of CD‐IP alone. Therefore, these additives may be used as supplements to inhibit methane production as well as to improve rumen fermentation and animal performance.