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Serum osteocalcin in donkeys as evaluated with an equine‐specific radioimmunoassay
Author(s) -
Carstanjen B.,
Amory H.,
Youssao I.,
Remy B.
Publication year - 2004
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/j.1439-0396.2004.00443.x
Subject(s) - donkey , radioimmunoassay , serial dilution , equidae , medicine , osteocalcin , endocrinology , biology , alkaline phosphatase , biochemistry , pathology , ecology , paleontology , alternative medicine , enzyme
Summary The purpose of this study was to validate an equine‐specific osteocalcin (OC) radioimmunoassay (RIA) for use in donkeys and to establish age‐related changes in serum OC concentrations in healthy donkeys. Serial dilutions of donkey serum showed parallelism with standard curves obtained with the equine‐specific OC RIA. There was a tight linear regression between donkey serum OC values obtained with the equine specific OC RIA and a commercially available bovine‐specific OC RIA. Serum OC levels of 27 healthy donkeys, analysed with the equine‐specific OC RIA, showed a tight negative logarithmic regression with age. Least square means and standard error of serum OC values were 67 ± 10 μ g/l in 0.6–4‐year‐old donkeys (group 1), 32 ± 9 μ g/l in 5–9‐year‐old donkeys (group 2) and 15 ± 11 μ g/l in >9‐year‐old donkeys (group 3). Serum OC values were significantly (p < 0.01) higher in group 1 than in groups 2 and 3. The equine‐specific OC RIA may be a useful and practical tool to assess bone metabolism and skeletal diseases in donkeys.