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Analysis of genetic variability in the Czech Dachshund population using microsatellite markers
Author(s) -
Přibáňová M.,
Horák P.,
Schröffelová D.,
Urban T.,
Bechyňová R.,
Musilová L.
Publication year - 2009
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2008.00772.x
Subject(s) - microsatellite , czech , loss of heterozygosity , biology , population , genetics , polymerase chain reaction , allele , genetic variation , veterinary medicine , medicine , gene , linguistics , philosophy , environmental health
Summary The primary goal of this study was to analyse genetic variation within and among six Dachshund varieties in the Czech Republic using 10 microsatellites from StockMarks ® Paternity Polymerase Chain Reaction (PCR) Typing kit. A total of 632 Dachshunds were sampled – 99 Standard Smooth‐haired, 72 Standard Long‐haired, 272 Standard Wire‐haired, 42 Miniature Smooth‐haired, 73 Miniature Long‐haired and 74 Miniature Wire‐haired. Average observed heterozygosity and polymorphic information content ranged in particular Dachshund varieties between 0.58–0.70 and 0.57–0.64, respectively. Subsequent analysis of the population structure ( F ‐statistics; Nei’s genetic identity) showed that Standard Dachshunds shared allele frequencies most closely with their miniature equivalents, and smooth coat type is closer to Wire‐haired coat type than to the Long‐haired one. Analysis of molecular variance revealed that 11.81% of the total variance occured among varieties. The value of combined exclusion probability (0.9955) validated usefulness of this panel of microsatellites for parentage verification in all analysed populations. We carried out 234 parentage verifications with 1.28% cases where parentage did not match.