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Structural analysis and haplotype diversity in swine LEP and MC4R genes
Author(s) -
D'Andrea M.,
Pilla F.,
Giuffra E.,
Waddington D.,
Archibald A.L.
Publication year - 2008
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2007.00689.x
Subject(s) - haplotype , biology , genetics , gene , candidate gene , genetic variation , melanocortin 4 receptor , phylogenetic tree , allele , genetic diversity , livestock , melanocortin , population , receptor , medicine , ecology , environmental health
Summary Knowledge about structural variation of candidate genes could be important to improve breeding selection scheme and preserve genetic variability in livestock species. Leptin ( LEP ) and melanocortin‐4 receptor ( MC4R ) genes are involved in the energetic pathway and are obvious candidate genes for fatness. By sequencing LEP and MC4R genes in 72 pigs belonging to lean (Large White and Duroc), fat (Meishan and Casertana) breeds and also Wild Boar, 98 polymorphic sites, of which 91 were novel, were found in the Leptin sequence while only the previously described mutation was found in the MC4R gene. A total of 18 LEP haplotypes were observed and their distribution was unequal among the breeds. The phylogenetic analysis showed two haplotype branches distinguishing between lean and fat breeds.