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Characterization of the porcine insulin‐like growth factor‐binding protein, acid‐labile subunit gene: full‐length cDNA and DNA sequence, polymorphisms and expression profile
Author(s) -
Li S.,
Ren J.,
Huang L.
Publication year - 2007
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2007.00645.x
Subject(s) - biology , exon , complementary dna , microbiology and biotechnology , gene , intron , peptide sequence , nucleic acid sequence , sequence analysis , genetics
Summary Insulin‐like growth factor‐binding protein acid‐labile subunit (IGFALS) is an important glycoprotein in the circulation complex for insulin‐like growth factors‐I and ‐II (IGFs). So far only a partial porcine IGFALS sequence is available. We herein report the isolation, characterization, polymorphism identification and expression analysis of the porcine IGFALS gene. A 2000‐bp full‐length cDNA sequence was determined with 5′‐ and 3′‐Rapid Amplification of cDNA End (RACE) assay. It contains a 1821‐bp open reading frame encoding a protein of 606 amino acids with a calculated molecular mass of 66 kDa and a theoretical isoelectric point of 6.89. The deduced IGFALS protein shares high identity (70–80%) with other mammalian IGFALS. We also obtained a full‐length genomic DNA sequence of the gene. Similar to the other mammalian orthologues, particularly in terms of exon size and exon/intron boundaries, the porcine IGFALS gene spans a transcription unit of 2990 bp, consisting of two exons and one intron. Three single nucleotide polymorphisms (synonymous mutations) were identified in the DNA sequence. RT‐PCR assays indicate that IGFALS is expressed ubiquitously in pigs and transcripts are particularly abundant in the liver, lung, white adipose tissue, prostate, epididymis, thyroid and bladder.