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Application of bovine microsatellite markers for genetic diversity analysis of European bison ( Bison bonasus )
Author(s) -
Roth T.,
Pfeiffer I.,
Weising K.,
Brenig B.
Publication year - 2006
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2006.00613.x
Subject(s) - microsatellite , biology , genetic diversity , loss of heterozygosity , multiplex polymerase chain reaction , genetics , allele , multiplex , heterologous , locus (genetics) , zoology , polymerase chain reaction , gene , population , medicine , environmental health
Summary In this study, the cross‐amplification of a commercial multiplex set of 11 cattle ( Bos taurus ) microsatellites was tested on a panel of 35 European bison ( Bison bonasus ) individuals. After polymerase chain reaction optimization, all loci cross‐amplified successfully in investigated bisons. Number of alleles and observed and expected heterozygosity per locus are in the range of 2–4, 0.086–0.629 and 0.288–0.621 respectively. The availability of a heterologous set of multiplexed microsatellite markers derived from cattle opens an avenue for collecting profound genetic data for efficient conservation management strategies of the European bison.