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SNP identification, linkage and radiation hybrid mapping of the porcine lamin A/C ( LMNA ) gene to chromosome 4q
Author(s) -
Wagenknecht D.,
Stratil A.,
Bartenschlager H.,
Van Poucke M.,
Peelman L.J.,
Majzlík I.,
Geldermann H.
Publication year - 2006
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2006.00591.x
Subject(s) - lmna , genetics , biology , single nucleotide polymorphism , genetic linkage , lamin , chromosome , gene , microbiology and biotechnology , genotype
Summary The lamins are components of nuclear lamina and they have a profound influence on nuclear structure and functions. They are encoded by three genes, LMNA , LMNB1 and LMNB2 . A genomic fragment of the porcine LMNA gene (822 bp; from exons 7 to 9) was amplified by polymerase chain reaction and comparatively sequenced. Four single nucleotide polymorphisms (SNPs) were identified in intronic sequences: G 162 A , G 208 A , T 367 G and C 618 T . The SNPs are within the restriction sites for enzymes Bsh 1236I, Hpa II, Alu I and Bsh 1236I respectively. Allele frequencies at SNPs G 208 A , T 367 G and C 618 T were determined by using eight pig breeds. Linkage analysis in the Hohenheim Meishan × Piétrain family placed the LMNA gene in the chromosome 4q linkage group, between MEF2D and GBA ( MEF2D – 3.0 cM – LMNA – 0.2 cM – GBA ). In radiation hybrid mapping LMNA was most significantly linked to SW270 on chromosome 4 (39 cR; LOD = 7.86). The LMNA gene is located in the quantitative trait loci region for some carcass traits on chromosome 4q.