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Cytogenetic mapping of 11 functional genes to chicken chromosomes by fluorescence in situ hybridization
Author(s) -
Yuan J.,
Zhao Y.,
Zhang L.,
Li N.
Publication year - 2006
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2006.00571.x
Subject(s) - biology , fluorescence in situ hybridization , telomere , genetics , karyotype , microchromosome , bacterial artificial chromosome , gene , chromosome , in situ hybridization , microbiology and biotechnology , comparative genomic hybridization , metaphase , genome , gene expression
Summary With chicken bacterial artificial chromosome (BAC) DNA as probes, 11 non‐assigned functional genes were localized to chicken chromosomes 1 or 2 by fluorescence in situ hybridization (FISH). The 11 genes and their chromosomal positions are as follows: ALVEB5 , 1p26‐24; ACO2 , 1p16‐14; HSP108 , 1p14‐13; CD4 , 1q11; HSD3B; 1q11; SOD1 , 1q14‐21; LAMP1 , 1q24‐31; P2Y5 , 1q35‐36; EN2 , 2p31‐24; NPY , 2p14‐13 and CA2 , 2q31‐32. Metaphase chromosome spreads used for hybridization were prepared from embryonic chicken fibroblast cultures. The gene position was identified according to the international standardized G‐banded karyotype of chicken by measuring the relative fractional length from the telomere of the p‐arm to the hybridization signal (FLpter). The 11 genes mapped newly will enrich the cytogenetic map and serve as additional anchor markers for integrating the cytogenetic map with the genetic map of chicken.