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Verwendung von genetischen Markern für die Diagnostik von Zell‐Chimärismus beim Lamm
Author(s) -
Rejduch B.,
KozubskaSobocińska A.,
Radko A.,
Rychlik T.,
Słota E.
Publication year - 2004
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2004.00456.x
Subject(s) - biology , microsatellite , fluorescence in situ hybridization , litter , chromosome , genetics , microbiology and biotechnology , population , metaphase , quadruplets , genetic marker , andrology , gene , pregnancy , allele , demography , sociology , medicine , gestation , agronomy
Summary A total of 181 Romanov lambs, including 84 pairs of twins, three litters of triplets and one litter of quadruplets, were investigated. Erythrocyte antigens belonging to six blood group systems were determined with 16 reagents. Six microsatellite loci BMS360 , INRA123 , McM42 , CSSM66 , ETH225 and TGLA53 were used to genotype the lambs with automated DNA sizing technology. For cytogenetic diagnosis of leucocyte chimerism, the fluorescence in situ hybridization (FISH) was applied using bovine X and Y chromosome painting probes. The blood‐group typing of lambs from twin and multiple pregnancies allowed us to detect nine cases of erythrocyte chimerism in the investigated population. The analysis of microsatellite DNA sequences showed the presence of cell chimerism in 13 animals. The hybridization of a bovine X and Y chromosome‐specific probe resulted in two yellow brightly luminescent signals in 54,XX cells, and one yellow and one blue signal in 54,XY cells helped us diagnose chromosomal chimerism in seven animals.