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A G > C transversion within the primer binding site causes a ‘null’ allele of the microsatellite T2201 in the White Shepherd breed
Author(s) -
Pieńkowska A.,
Schelling C.
Publication year - 2001
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2001.00269.x
Subject(s) - transversion , null allele , genetics , biology , microsatellite , allele
In the course of a routine paternity test for White Shepherd dogs using microsatellite T2201 an apparent non‐Mendelian inheritance was seen. It could be shown that the observed phenomenon was caused by a G > C transversion within the reverse primer binding site of one maternal allele leading itself to a ‘null’ allele. Using an alternative primer complementing for the transversion the ‘null’ allele and all other known alleles of the microsatellite T2201 in White Shepherd dogs can be amplified under standard polymerase chain reaction conditions. Although the frequency of this ‘null’ is very low (0.08) we recommend the use of the alternative primer for paternity testing and related applications (e.g. genotype data bank) in this breed.