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Assignment of new loci to river buffalo chromosomes confirms the nature of chromosomes 4 and 5
Author(s) -
NAHAS By S. M. EL,
HONDT H. A.,
SOUSSA S. F.,
GHOR A. EL,
HASSAN A. A.
Publication year - 1999
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.1999.00167.x
Subject(s) - synteny , biology , homology (biology) , genetics , chromosome , karyotype , metaphase , autosome , cytogenetics , hybrid , homologous chromosome , gene , botany
Rapid development of the river buffalo physical map can be achieved by coupling its development to that of the cattle gene map. Syntenic conservation between cattle and buffalo has been demonstrated, mainly using somatic cell hybrids ( de H ondt et al. 1991; E l N ahas et al. 1993, 1996, 1998; de H ondt et al. 1997; E l N ahta 1996; O raby et al. 1977), and by using in situ hybridization as reviewed by I annuzzi (1997). G‐ and R‐banding comparisons between cattle (2n = 60) and river buffalo (2n = 50) chromosomes have revealed a large number of banding homologies between the two species, both at early‐metaphase (G upta and R ay ‐C haudhury 1978; D i B erardino et al. 1981) and prometaphase stages (I annuzzi et al. 1990). Banding homology indicates that the five river buffalo biarmed pairs originate from centric fusion translocation between two of ten homologous cattle autosomes, which is very supportive of the hypothesis that both species have a common ancestor (W urster and B enirschke 1968). Based on cytological analysis and banding homology between cattle and buffalo chromosomes, the five biarmed chromosomes of the river buffalo BBU1, BBU2, BBU3, BBU4, BBU5 were thought to originate from fusion of cattle chromosome (BTA) 1/25; 2/23; 8/19; 5/28; and 16/29 respectively (I annuzzi et al. 1990; R eport of the C ommittee for the S tandardization of B anded K aryotopes of the R iver B uffalo 1994). However, the analysis of synteny between molecular markers assigned to different cattle syntenic groups demonstrated that BBU1 results from fusion of BTA 1 and 27 rather than 1 and 25 (E l N ahas et al. 1977). This called for expanding the analysis of syntenic relationships between marker loci to confirm the nature of the other biarmed buffalo chromosomes. The purpose of this study is to test synteny between markers in buffalo and to confirm the nature of the biarmed buffalo chromosomes 4 and 5, using marker loci and somatic cell hybrids.