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Interactive Effects of High Boron and NaCl Stresses on Subcellular Localization of Chloride and Boron in Wheat Leaves
Author(s) -
Masood S.,
Wimmer M. A.,
Witzel K.,
Zörb C.,
Mühling K. H.
Publication year - 2012
Publication title -
journal of agronomy and crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 74
eISSN - 1439-037X
pISSN - 0931-2250
DOI - 10.1111/j.1439-037x.2011.00501.x
Subject(s) - salinity , shoot , apoplast , chemistry , hydroponics , toxicity , boron , dry weight , transpiration , chloride , symplast , horticulture , germination , agronomy , zoology , biology , cell wall , biochemistry , photosynthesis , organic chemistry , ecology
Salinity and boron (B) toxicity often occur simultaneously and may have interactive effects on plant responses. This study aims at further investigating long‐term effects of salinity, B toxicity and their combination on wheat plants. Plants grown in hydroponics were treated with 2.5 μ m H 3 BO 3 (control), 75 m m NaCl, 200 μ m H 3 BO 3 or 75 m m NaCl + 200 μ m H 3 BO 3 and were analysed on a subcellular level 6 weeks after germination. Shoot fresh and dry weight (DW), water content, transpiration rate and osmolality were reduced, while Na + and Cl − concentrations increased under salinity stress. However, Cl − was reduced in all compartments by an additional application of B toxicity. At adequate B supply, NaCl increased apoplastic and symplastic soluble B concentrations, whereas the total B content remained unchanged. At high B level, however, soluble and total B was reduced by additional salt stress. Despite this antagonistic effect of a combined salt and B toxicity stress on Cl − and B concentrations, an additive effect was observed regarding shoot fresh weight reduction. Our results confirm an alleviating effect of the combined stresses on toxic ion concentrations, which did not prevent additive growth reductions.

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