Premium
Effect of the Russian Wheat Aphid on the Composition and Synthesis of Water Soluble Proteins in Resistant and Susceptible Wheat
Author(s) -
Westhuizen A. J.,
Botha F. C.
Publication year - 1993
Publication title -
journal of agronomy and crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 74
eISSN - 1439-037X
pISSN - 0931-2250
DOI - 10.1111/j.1439-037x.1993.tb01091.x
Subject(s) - russian wheat aphid , cycloheximide , aphid , biology , infestation , agronomy , poaceae , homoptera , composition (language) , rhopalosiphum padi , gene , protein biosynthesis , aphididae , botany , biochemistry , pest analysis , linguistics , philosophy
The effect of Russian wheat aphid ( Diuraphis noxia) infestation on polypeptide composition and protein synthesis in the presence or absence of chloramphenicol and cycloheximide were studied in genetically comparable wheat ( Triticum aestvum) resistant (cv. PI 137739/5* Tugela) and susceptible (cv. Tugela) to the aphid. The aim is to gain information of molecular nature on the resistance phenomenon which may promote future breeding programmes. In the absence of feeding aphids polypeptide profiles of the two wheat lines were similar indicating the absence or presence at low levels of a constitutive resistance factor. Aphid infestation induced enhanced expression of certain genes in the resistant wheat only. A 100 kD nuclear encoded polypeptide is strongly induced in the resistant wheat. It is also evident that the synthesis of a 56 kD organel encoded polypeptide is suppressed by the feeding aphids in the susceptible wheat.