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Seed Protein Electrophoresis for Varietal Identification in Rice ( Oryza sativa L.)
Author(s) -
Yupsanis T.,
Moustakas M.,
Karakoli S.
Publication year - 1992
Publication title -
journal of agronomy and crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 74
eISSN - 1439-037X
pISSN - 0931-2250
DOI - 10.1111/j.1439-037x.1992.tb00984.x
Subject(s) - prolamin , oryza sativa , globulin , japonica , biology , japonica rice , botany , poaceae , gel electrophoresis , agronomy , polyacrylamide gel electrophoresis , storage protein , biochemistry , enzyme , gene , immunology
Albumins, prolamins and globulins were extracted from milled rice of long, medium and short grain varieties Japonica type and analyzed by native PAGE and SDS‐PAGE. A computer aided analysis of protein patterns was employed in order to estimate the best electrophoresis method to differentiate between rice varieties. The patterns of SDS‐electrophoresis of albumins and globulins showed the biggest differences in all types of rice grains and allowed the identification of the rice varieties examined, since for each variety the pattern was different. Therefore, the technique of SDS‐electrophoresis of albumins and globulins is recommended as more suitable to distinguish among closely related varieties and can be used by rice breeders in order to characterize various varieties and pick the most diverse for breeding purposes.