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Electrophoretic and Serological Studies of the Seed Proteins of some Members of the Subfamily Papilionoideae
Author(s) -
Sammour R. H.
Publication year - 1987
Publication title -
journal of agronomy and crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 74
eISSN - 1439-037X
pISSN - 0931-2250
DOI - 10.1111/j.1439-037x.1987.tb00102.x
Subject(s) - vicilin , legumin , subfamily , storage protein , biology , electrophoresis , immunodiffusion , serology , globulin , botany , biochemistry , genetics , gene , immunology , antibody
Electrophoretic and Immunochemical relationships amongst storage proteins of some tribes of subfamily Papilionoideae have been investigated on the basis of their legumins and vicilins subunit compositions. SDS‐PAGE, immunodiffusion, and “Western” blotting technique are the technique which were used in this study. Electrophoretically, it has been found that each tribe has a distinctive electrophoretic pattern. Immunochemically, legumin and vicilin‐like proteins were detected for the first time in some members of Phasealeoe, Glycineae, Cajaneae , and Diocleae.