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Microspatial genetic diversity of Dothistroma septosporum
Author(s) -
Kraj W.,
Kowalski T.
Publication year - 2013
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/j.1439-0329.2012.00791.x
Subject(s) - biology , mantel test , genetic diversity , geographical distance , genetic distance , genetic variation , analysis of molecular variance , population , microsatellite , heritability , veterinary medicine , genetic structure , genetics , evolutionary biology , statistics , mathematics , allele , demography , medicine , sociology , gene
Summary The distribution of genetic diversity in a local population of D othistroma septosporum was determined on a microspatial geographical scale. The study was conducted in a seed orchard of P inus nigra divided into four plots, each of 0.84 ha in area. Ninety‐two isolates were obtained from needles with red band needle blight symptoms. Molecular variance, Mantel test and autocorrelation spatial analyses were performed with the use of random amplified microsatellites markers to test the null hypothesis of a random distribution of genotypes. The groups of isolates from individual plots demonstrated small differences as regards intrapopulation variation. The mean contribution of polymorphic loci for isolate groups was almost 77%, genetic diversity 0.270, while the Shannon coefficient was 0.389. The M antel test demonstrated a significant, positive correlation between H uff's genetic distance and geographical distance. Significant spatial genetic structure was detected with autocorrelation coefficients being significant in the first two distance classes up to about 8–12 m.