Premium
Fast molecular detection of Pseudomonas syringae pv. aesculi in diseased horse chestnut trees
Author(s) -
Schmidt O.,
Moreth U.,
Dujesiefken D.,
Stobbe H.,
Gaiser O.
Publication year - 2009
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/j.1439-0329.2009.00595.x
Subject(s) - pseudomonas syringae , biology , aesculus hippocastanum , dna gyrase , pathovar , polymerase chain reaction , microbiology and biotechnology , pseudomonadaceae , pathogen , pseudomonas , bacteria , botany , gene , genetics , escherichia coli
Summary A molecular technique was used to detect the bacterium Pseudomonas syringae pv. aesculi in horse chestnut trees ( Aesculus hippocastanum ), affected by the recently recognized European ‘ Pseudomonas horse chestnut bark disease’. The technique helped identify the pathogen within 6 h of sample preparation including DNA extraction, polymerase chain reaction (PCR) and electrophoresis until gel documentation. PCR primer pairs derived from the gyrase B gene sequence were used. Because of the great similarity in the gyrase B gene sequences of the numerous closely related P. syringae pathovars, the primers were not only totally specific to the pathovar aesculi , but also detected a few other pathovars. The assumption that other bacteria should not occur at least near to a necrotic lesion of a horse chestnut tree was corroborated by sequence identity of the PCR products obtained with the gyrase B gene sequence of P. syringae pv. aesculi . Koch’s postulates were fulfilled for an isolate of P. syringae pv. aesculi obtained from a diseased horse chestnut tree sampled in Hamburg in 2007.