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Microbial communities in roots of Pinus sylvestris seedlings with damping‐off symptoms in two forest nurseries as determined by ITS1/2 rDNA sequencing
Author(s) -
Kwaśna H.,
Bateman G. L.
Publication year - 2009
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/j.1439-0329.2008.00583.x
Subject(s) - biology , damping off , botany , basidiomycota , fusarium oxysporum , ascomycota , root rot , fungus , ribosomal dna , ectomycorrhizae , fusarium , microbial population biology , rhizoctonia , phylogenetics , rhizoctonia solani , bacteria , biological pest control , mycorrhiza , symbiosis , gene , biochemistry , genetics
Summary A methodological molecular procedure, which included extraction and cloning of the ITS1/2 rDNA of root‐associated organisms with subsequent transformation and sequencing of representative clones, was effective for detection, discrimination and determination of the frequency of the main damping‐off pathogens in roots of Pinus sylvestris seedlings growing in different forest‐tree nursery soils and exhibiting different rates of disease progress. Roots exhibiting slower damping‐off progression were colonized by Fusarium oxysporum , Neonectria radicicola (Ascomycota) and Pythium spp. (Oomycota), which comprised 50% of the microbial community. Roots exhibiting faster damping‐off progression were dominated by Thanatephorus cucumeris (Basidiomycota), which comprised 80% of the microbial community . The microbial community was more diverse in roots with slower damping‐off progression (14 species) than in roots with faster disease progression (seven species).