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Accumulation of a porin‐like mRNA and a metallothionein‐like mRNA in various clones of Norway spruce upon long‐term treatment with ozone
Author(s) -
Buschmann K.,
Etscheid M.,
Riesner D.,
Scholz F.
Publication year - 1998
Publication title -
european journal of forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 0300-1237
DOI - 10.1111/j.1439-0329.1998.tb01186.x
Subject(s) - cdna library , biology , suppression subtractive hybridization , complementary dna , microbiology and biotechnology , ozone , clone (java method) , porin , messenger rna , metallothionein , picea abies , gene , botany , genetics , chemistry , organic chemistry , escherichia coli , bacterial outer membrane
Summary In a long‐term study six Norway spruce (Picea abies) clones from two different provenance regions were exposed to 150/μg/m 3 and 300μg/m 3 ozone in open top chambers. Differential screening of subtractive cDNA libraries made from mRNA of ozone‐treated trees identified several cDNA clones of ozone‐responsive genes, two of which were studied in more detail. One cDNA clone corresponded to a porin‐like mRNA, the other to a metallothionein‐like mRNA. Under moderate ozone exposure the accumulation of these two mRNAs was already detectable before damage symptoms became visible. The spruce clones varied in the level of accumulation for the mRNAs. This is discussed under the aspect of ozone‐induced early senescence rather than a defense reaction suppressing symptoms, because the two phenotypically most ozone‐‘tolerant’ spruce clones showed the lowest levels of accumulation for both mRNAs.