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Characterization of Cryphonectria parasitica strains by random amplified polymorphic DNA (RAPD) technique and conventional methods
Author(s) -
Wronski R.,
Kudera U.,
Wlhelm E.
Publication year - 1997
Publication title -
european journal of forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 0300-1237
DOI - 10.1111/j.1439-0329.1997.tb01360.x
Subject(s) - cryphonectria , biology , rapd , genetics , dna , chestnut blight , polymorphism (computer science) , evolutionary biology , botany , genetic diversity , genotype , gene , virulence , population , demography , sociology , fungus
Summary In recent years damage to Austrian Castanea sativa populations caused by Cryphonectria parasitica has increased. A total of 34 isolates out of 13 observation plots of this phytopathogen in the south‐east of Austria were examined for laccase activity, virulence, number of vegetative compatibility groups and for the existence of hypovirulence associated dsRNA. Furthermore, the applicability of the random amplified polymorphic DNA (RAPD) technique for investigating population structure was tested. Small dsRNA fragments were found in two Austrian strains. The same two strains exhibited reduced virulence. Seven vegetative compatibility groups were found in the areas examined. RAPD analysis proved to be an efficient method for distinguishing between different C. parasitica genotypes.