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Immunocytochemical localization of pathogenesis‐related proteins in roots of Norway spruce infected with Heterobasidion annosum
Author(s) -
Asiegbu F. O.,
Denekamp M.,
Daniel G.,
Johansson M.
Publication year - 1995
Publication title -
european journal of forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 0300-1237
DOI - 10.1111/j.1439-0329.1995.tb00332.x
Subject(s) - heterobasidion annosum , chitinase , immunogold labelling , hypha , biology , root rot , glucanase , labelling , microbiology and biotechnology , cell wall , seedling , botany , inoculation , picea abies , ultrastructure , enzyme , biochemistry , horticulture
Summary The occurrence and accumulation of β‐1, 3‐glucanase and chitinase in seedling roots of spruce ( Picea abies ) following challenge by the root‐rot pathogen Heterobasidion annosum were studied. Chitinase activity increased 2–3 fold following inoculation, whereas no significant increase in the activity levels of glucanase was recorded during infection. With TEM immunogold labelling, the enzymes were localized in protein aggregates in host tissues and in the cell walls of intercellular hyphae. Gold particles were sparse and irregularly distributed within host‐cell walls. Only minor labelling was observed on hyphal walls coated with electron‐dense materials. The labelling intensity increased with infection time and was always higher than in non‐infected seedling roots. When this experiment was repeated using root samples inoculated with the saprophyte Phlebiopsis gigantea , a similar labelling pattern was observed. The cross reactivity of antisera raised against sugar‐beet chitinase and glucanase with spruceroot enzyme extracts was demonstrated using dot‐blot assays and ELISA.