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Comparaison des activités phosphatases ?Homobasidiomycètes ectomycorhiziens en culture in vitro
Author(s) -
Mousain D.,
Bousquet Nadine,
Polard Catherine
Publication year - 1988
Publication title -
european journal of forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 0300-1237
DOI - 10.1111/j.1439-0329.1988.tb00217.x
Subject(s) - mycelium , pisolithus , phosphatase , acid phosphatase , incubation , biology , phosphate , alkaline phosphatase , in vitro , sodium , ectomycorrhiza , biochemistry , chemistry , mycorrhiza , botany , enzyme , symbiosis , bacteria , genetics , organic chemistry
Comparison of phosphatase activities in ectomycorrhizal Homobasidiomycetes cultured in vitro . Phosphatase activities, measured on intact mycelia (accessible activities) or on mycelial homogenates (total activities), were compared in twelve ectomy corrhizal strains of Homobasidiomycetes ( Hebeloma cylindrosporum, Laccaria laccata; Suillus bellini; S. bovinus; S. granulatus; S. luteus, Pisolithus arhizus ). The effects of the following factors were examined: (i) two orthophosphate concentrations in the culture medium; (ii) two incubation substrates (sodium p ‐nitrophenylphosphate or sodium phytate). Phosphatase activities were highest when mycelial homogenates were incubated in the presence of p ‐nitrophenyl phosphate after culturing the mycelia on medium slightly deficient in orthophosphate, particularly in the case of one S. granulatus isolate.