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Effect of sulfur‐dioxide on protein‐SH in needles of Picea abies 1
Author(s) -
Grill D.,
Esterbauer H.,
Scharner M.,
Felgitsch Ch.
Publication year - 1980
Publication title -
european journal of forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 0300-1237
DOI - 10.1111/j.1439-0329.1980.tb00038.x
Subject(s) - picea abies , chemistry , glutathione , reagent , sulfur dioxide , structural protein , sulfur , biochemistry , botany , biology , organic chemistry , enzyme , gene
The effect of SO 2 on the SH‐groups of proteins in needles of Picea abies is studied. The content of total protein SH and glutathione is estimated with 5.5′‐Dithio‐bis‐nitrobenzoic‐acid (Ellman reagent). In addition structural protein SH is measured microspectrophotometrically in sections stained with the SH reagent, mercurochrome. The results indicate that GSH and also total protein SH and structural protein SH are increased in needles of Picea abies growing in SO 2 polluted areas. Compared to needles of trees in relatively unpolluted areas average increases of 2. 33 (GSH), 1.22 (total protein SH) and 1.55 (structural protein SH) are found.