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Cytoprotective effects of fruit pulp of E ugenia jambolana on H 2 O 2 ‐induced oxidative stress and apoptosis in rat L eydig cells in vitro
Author(s) -
Anand H.,
Misro M. M.,
Sharma S. B.,
Prakash S.
Publication year - 2013
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2012.01323.x
Subject(s) - oxidative stress , apoptosis , glutathione , superoxide dismutase , caspase 3 , catalase , biology , chemistry , microbiology and biotechnology , biochemistry , programmed cell death , enzyme
Summary This study was undertaken to investigate the cytoprotective effect of the fruit pulp of E ugenia jambolana (50–250 μg ml −1 ) against the damage induced by H 2 O 2 (100 μ m ) exposure to L eydig cells in vitro . Cell survival with extract was found comparable to similar effects by N ‐acetyl‐ l ‐cysteine. H 2 O 2 ‐induced rise in thiobarbituric acid reactive substance formation and decline in the activity and expression of antioxidant enzymes like superoxide dismutase, catalase and glutathione‐s‐transferase were effectively checked. Cellular glutathione and total antioxidant capacity demonstrated significant improvement. The increase in expression of inducible nitric oxide ( NO ) synthase leading to NO production was successfully countered. Co‐treatment of the extract helped in the down‐regulation of caspase‐3 and poly‐ ADP ‐ribose polymerase resulting in a significant reduction in L eydig cell apoptosis induced by H 2 O 2 . Upstream marker proteins of extrinsic (caspase‐8, F as, F as L ) and intrinsic (caspase‐9) pathway of metazoan apoptosis were identically down‐regulated. The B cl‐2 family of proteins, though, remained unaffected. The extract also positively modulated the other marker proteins like c‐ J un NH 2 ‐terminal kinase, p38, A kt, nuclear factor‐κ B , c‐ F os, cellular FLICE ‐inhibitory protein, cyclooxygenase‐2 and p53. Taken together, the above‐mentioned findings establish the anti‐oxidative and anti‐apoptotic potency of the extract that ameliorates the H 2 O 2 ‐induced adverse effects on rat L eydig cells in vitro .