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Effect of testosterone and frequent low‐dose sildenafil/tadalafil on cavernous tissue oxidative stress of aged diabetic rats
Author(s) -
Mostafa T.,
Rashed L.,
Kotb K.,
Taymour M.
Publication year - 2012
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2012.01294.x
Subject(s) - tadalafil , sildenafil , medicine , endocrinology , cyclic guanosine monophosphate , erectile dysfunction , oxidative stress , streptozotocin , diabetes mellitus , glutathione peroxidase , testosterone (patch) , superoxide dismutase , nitric oxide
Summary This study aimed to assess the effect of testosterone ( T ) administration and chronic low‐dose sildenafil/tadalafil on cavernous tissue oxidative stress ( OS ) of aged diabetic rats. In all, 140 Sprague‐Dawley aged rats were subdivided into the following: controls; streptozotocin ( STZ )‐induced diabetic rats; diabetic rats injected with T every 4 weeks; diabetic rats on sildenafil orally daily; diabetic rats on T and daily sildenafil; diabetic rats on tadalafil orally every other day; diabetic rats on T and tadalafil; diabetic rats on alternate sildenafil/tadalafil; and diabetic rats on alternate sildenafil/tadalafil with T . After 12 weeks, the rats were euthanised where in dissected cavernous tissues malondialdehyde ( MAD ), glutathione peroxidase ( GPx ) and cGMP (cyclic guanosine monophosphate) were estimated. Compared with controls, aged diabetic rats demonstrated significant increase in cavernous tissue MDA and significant decrease in GP x and cGMP where diabetic rats injected with T had marked improvement of these parameters. Diabetic rats on sildenafil, tadalafil or alternate sildenafil/tadalafil demonstrated significant increased cavernous tissue GP x, cGMP and decreased cavernous MDA that was further improved when supplemented with T . It is concluded that frequent low‐dose use of sildenafil and/or tadalafil supplemented with T has a marked impact on ameliorating cavernous OS in aged diabetic rats.