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Oestrogen receptors in the developing rat prostate
Author(s) -
ElShafei M. D. M.,
Mostafa M. E. A.,
Mostafa T.
Publication year - 2011
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2009.01027.x
Subject(s) - prostate , immunohistochemistry , haematoxylin , endocrinology , medicine , androgen receptor , h&e stain , genitourinary system , androgen , receptor , pathology , andrology , biology , prostate cancer , hormone , cancer
Summary This study aimed to illustrate the histological picture of the developing rat prostate and to localise the oestrogen receptors (ERs) distribution. Fifty male albino rats were divided into five equal subgroups: subgroup 1, rats aged 2 days (before morphogenesis of prostate from urogenital sinus); subgroup 2, rats aged 1 week (after early morphogenesis); subgroup 3, rats aged 2 weeks (early pubertal stage); subgroup 4, rats aged 4 weeks (late pubertal stage) and subgroup 5 rats aged 6 weeks (maturation stage). After the rats had been killed, the prostates were dissected and specimens were obtained from the anterior lobe. Paraffin sections of these specimens were subjected to haematoxylin and eosin stain, immunohistochemistry and quantitative immunohistohemistry for ERs distribution and the area of strong positive cytoplasmic immunoreactivity. The results demonstrated that ERs protein decreased gradually with increased age and that it decreased markedly with the onset of puberty. It is concluded that the prostate despite being an androgen dependent gland, depends greatly on oestrogen in its development in the prepubertal period.