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Quercetin protects hamster spermatogenic cells from oxidative damage induced by diethylstilboestrol
Author(s) -
Li G.,
Ma A.,
Shi W.,
Zhong X. H.
Publication year - 2010
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2009.00990.x
Subject(s) - quercetin , oxidative stress , lipid peroxidation , superoxide dismutase , glutathione , glutathione peroxidase , antioxidant , intracellular , andrology , chemistry , biology , biochemistry , pharmacology , medicine , enzyme
Summary Quercetin has been reported to be an efficient antioxidant which protects chicken spermatogonial cells from oxidative damage through increasing intracellular antioxidants and decreasing lipid peroxidation. Exposure to diethylstilboestrol (DES) could cause reproductive damage in males, which is associated with oxidative stress. This study was conducted to investigate the protective effects of quercetin on DES‐induced oxidative damage in cultured hamster spermatogenic cells. The cells were treated with different concentrations of DES, and their growth status was observed under inverted microscope. The viability of spermatogenic cells was detected by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide (MTT). The contents of superoxide dismutase (SOD) in supernatants and glutathione peroxidase (GSH‐Px) in cells were detected with spectrophotography. The results showed that quercetin significantly inhibited the DES‐induced damage on spermatogenic cells, with the exception of the low‐dose group in which no significant difference was observed. The cell survival rate increased significantly in the middle‐ and high‐dose groups. The contents of SOD and GSH‐Px were significantly elevated after medication with quercetin ( P < 0.01). It can be concluded that quercetin protects spermatogenic cells against DES‐induced oxidative damage through increasing intracellular antioxidants and decreasing lipid peroxidation. Quercetin plays a very important role in ameliorating reproductive toxicity induced by environmental oestrogens.