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GnRH agonist induces apoptosis in seminiferous tubules of immature rats: direct gonadal action
Author(s) -
Peirouvi T.,
Salami S.
Publication year - 2010
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2009.00982.x
Subject(s) - buserelin , endocrinology , medicine , haematoxylin , agonist , apoptosis , terminal deoxynucleotidyl transferase , programmed cell death , biology , hormone , andrology , immunohistochemistry , tunel assay , receptor , biochemistry
Summary To investigate direct gonadal effect of buserelin as an agonist of gonadotrophin releasing hormone, the incidence of apoptotic cell death was measured. Thirty 25‐day‐old immature Wistar male rats were divided into two groups: treated and control rats. Treated rats were given 1.25 mg buserelin acetate/g body weight control rats received vehicle subcutaneously for 5 days. Formalin‐fixed paraffin embedded testicles were then investigated for the morphology of seminiferous tubules and occurrence of apoptosis using haematoxylin‐eosin staining and terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labelling respectively. Immaturity of rats was proved by the morphological characteristics of testis. In contrast to the control rats, significant increase of apoptotic cell death was found in buserelin‐treated rats. Apart from the well‐known pituitary‐testicular function of buserelin as an agonist of gonadotrophin releasing hormone, our findings suggest that it induces apoptotic cell death via direct gonadal action.