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A study of meiotic segregation of chromosomes in spermatozoa of translocation carriers using fluorescent in situ hybridisation
Author(s) -
Perrin A.,
Morel F.,
DouetGuilbert N.,
Le Bris M.J.,
Amice J.,
Amice V.,
De Braekeleer M.
Publication year - 2010
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2009.00951.x
Subject(s) - chromosomal translocation , meiosis , fluorescence , in situ hybridisation , biology , in situ , andrology , microbiology and biotechnology , genetics , chemistry , medicine , optics , physics , gene , organic chemistry
Summary In the infertile male population, there is a 2–20‐time higher probability of having a structural chromosomal abnormality than in general population. Generally, these men have a normal phenotype but they can have sperm abnormalities. As they can produce a variable proportion of unbalanced gametes, it is important to evaluate the percentage of unbalanced chromosomal spermatozoa to assess the risk of injecting a chromosomally unbalanced gamete during ICSI procedure. We report here the meiotic segregation analysis of chromosomes in spermatozoa of 12 men with a balanced reciprocal translocation and 4 men with a Robertsonian translocation using a fluorescent in situ hybridisation analysis. The frequencies of normal or balanced spermatozoa ranged from 34.4% to 49.1% in balanced reciprocal translocation carriers. For Robertsonian translocation, the frequencies of normal or balanced spermatozoa ranged from 78.4% to 91.2%. These analyses allow us to define the orientation of genetic counselling according to the results of meiotic segregation obtained. As a last resort, it could then be discussed of the possibility of having recourse to donor spermatozoa or adoption.