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Effect of HO‐1 cDNA‐liposome complex transfer on erectile signalling of aged rats
Author(s) -
Abdel Aziz M. T.,
Mostafa T.,
Atta H.,
Mahfouz S.,
Wassef M.,
Fouad H.,
Kamel M.,
Rashed L.,
Sabry D.,
Mouhamed O.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2008.00911.x
Subject(s) - complementary dna , liposome , nitric oxide synthase , western blot , biology , microbiology and biotechnology , histopathology , nitric oxide , endocrinology , medicine , biochemistry , pathology , gene
Summary This work aimed to assess the efficacy of haeme oxygenase‐1 (HO‐1) cDNA‐liposome complex transfer as a mediator of erectile signalling in aged rats. One hundred and fifty aged white albino rats were equally divided into five groups: controls, rats receiving lipofectamine, rats receiving intracorporeal HO‐1 cDNA‐lipsome complex, rats receiving HO‐1 cDNA‐liposome complex plus nitric oxide synthase (NOS) inhibitor, and rats receiving HO‐1 cDNA‐liposome complex plus HO inhibitor. Six rats were killed from each group after 12, 24 and 48 h, and after1 and 2 weeks. In dissected cavernous tissues, the following were assessed: HO‐1 gene expression, Western blot for HO‐1, HO enzyme activity, cGMP and histopathology. The results showed that HO‐1 cDNA‐liposome complex transfer led to a significant increase in cavernous tissue HO‐1 protein, HO‐1 gene expression, HO enzyme activity and cGMP up to 1 week. NOS inhibition exhibited no effect on HO‐1 gene enhancement of cavernous tissue HO enzyme activity or cGMP, whereas inhibition of HO significantly decreased these parameters. Histopathology of cavernous tissue demonstrated a significant dilatation of helicine arteries in HO‐1 cDNA‐liposome complex treated group after 48 h compared with the controls. It is concluded that HO‐1 cDNA‐liposome complex transfer augments cavernous tissue cGMP with subsequent sinusoidal relaxation.