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Expression and function of ryanodine receptors in rabbit penile corpus cavernosum smooth muscle cells
Author(s) -
Liu H. G.,
Zhu G. Y.,
Wang Y. H.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2008.00904.x
Subject(s) - ryanodine receptor , endocrinology , medicine , receptor , reverse transcription polymerase chain reaction , biology , microbiology and biotechnology , chemistry , andrology , messenger rna , biochemistry , gene
Summary This study aimed to investigate the expression and function of ryanodine receptors (RyRs) in rabbit penile corpus cavernosum smooth muscle (CCSM) cells. New Zealand white rabbit CCSM cells were cultured by primary tissue culture and identified by immunofluorescence technique. mRNA of three RyRs subunits in cultured CCSM cells was detected by reverse transcription polymerase chain reaction (RT‐PCR). After excitation by noradrenaline, the concentration of Ca 2+ in CCSM cells was detected by laser scanning confocal microscopy. It was found that only the RyRs1 subunit is expressed in CCSM by RT‐PCR. The CCSM cells were divided into two groups: Group A, CCSM cells + 10 μmol l −1 noradrenaline and Group B, CCSM cells + 50 μmol l −1 procaine 10 μmol l −1 noradrenaline. Compared with the base level and the level after noradrenaline excitation, fluorescence intensity improved 44.10 ± 6.01% in the test group A ( n = 8) and 32.92 ± 4.92% ( n = 8) in the procaine control group B, respectively. There were statistically significant differences between group A and group B ( P < 0.01). It is concluded that RyRs1 subunit is expressed in CCSM cells and may contribute to regulating the cytoplasmic Ca 2+ level.