Premium
Blocking E‐selectin inhibits ischaemia–reperfusion‐induced neutrophil recruitment to the murine testis
Author(s) -
Celebi M.,
Paul A. G. A.
Publication year - 2008
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2008.00849.x
Subject(s) - testicular torsion , ischemia , p selectin , reperfusion injury , neutrophile , monoclonal antibody , andrology , apoptosis , flow cytometry , inflammation , cell adhesion molecule , immunology , biology , pharmacology , antibody , medicine , biochemistry , platelet , surgery , platelet activation
Summary Germ cell‐specific apoptosis that occurs after ischaemia–reperfusion (IR) of the murine testis is dependent on neutrophil recruitment to the testis and is dependent upon the cell adhesion molecule E‐selectin. In this study, we aimed to inhibit neutrophil recruitment to the IR‐induced testis. Mice were subjected to a 2‐h period of testicular torsion (ischaemia) followed by detorsion (reperfusion). Shortly after onset of reperfusion, mice received either a function‐blocking monoclonal anti‐mouse E‐selectin antibody (FBmAb) or isotype‐matched control antibody. Mice were killed 24 h after reperfusion and cells isolated from the testis were analysed for the presence of neutrophil infiltration by flow cytometry. Administration of FBmAb inhibited neutrophil recruitment to the IR‐induced testis dramatically. Therefore, blockage of E‐selectin may be a strategy to treat post‐ischaemic testis.