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Spermiogenesis defects in human: detection of transition proteins in semen from some infertile men
Author(s) -
Becker S.,
Soffer Y.,
Lewin L. M.,
Yogev L.,
Shochat L.,
Golan R.
Publication year - 2008
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2008.00844.x
Subject(s) - asthenozoospermia , andrology , semen , spermiogenesis , azoospermia , staining , flow cytometry , semen analysis , male infertility , biology , propidium iodide , spermatogenesis , microbiology and biotechnology , infertility , medicine , apoptosis , genetics , programmed cell death , pregnancy
Summary Semen samples from 60 infertile men were examined by flow cytometry following propidium iodide staining. Of these, 23 samples contained young haploid cells. Transition proteins (TP1 and/or TP2) were detected in 12 of these, using immunohistochemical staining. The presence of TPs in spermatids in semen indicates inhibition in the differentiation pathway from round spermatids to spermatozoa. Cells of this type were found in semen from patients with nonobstructive azoospermia, severe to extreme cases of oligozoospermia, asthenozoospermia and teratozoospermia.