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Y‐27632 improves the erectile dysfunction with ageing in SD rats through adjusting the imbalance between nNo and the Rho‐kinase pathways
Author(s) -
Gao B. H.,
Zhao S. T.,
Meng F. W.,
Shi B. K.,
Liu Y. Q.,
Xu Z. S.
Publication year - 2007
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2007.00782.x
Subject(s) - rho associated protein kinase , erectile dysfunction , endocrinology , medicine , erectile function , kinase , western blot , protein kinase a , chemistry , rho kinase inhibitor , biochemistry , gene
Summary We investigated whether the effect of Y‐27632 to improve the erectile function in SD rats was associated with the degree of the imbalance between nNOS and the Rho‐kinase pathways. Western blot analysis was used to evaluate nNOS and Rho‐kinase protein expression in 10 young and 10 old SD rats. Imbalance value between nNOS and Rho‐kinase protein levels was obtained by subtracting nNOS from Rho‐kinase. A 5‐V stimulus was given in SD rats before and after the administration of 200 nmol kg −1 of Y‐27632 intracavernosally and ICP/MAP was recorded. The improvement of erectile function induced by Y‐27632 was expressed as the margin of ICP/MAP after and before the administration of Y‐27632. In young rat group, the contents of nNOS and Rho‐kinase protein were 1.7 ± 0.15 and 1.8 ± 0.14 respectively. In old rat group, the nNOS protein decreased to 1 ± 0.15, and in contrast, the Rho‐kinase protein increased to 2.6 ± 0.2. The imbalance value between nNOS and Rho‐kinase was 0.2986 ± 0.1109 and 1.5961 ± 0.1206 in young and old rat groups. The improvement of erectile function induced by Y‐27632 was 0.0500 ± 0.0294 and 0.3420 ± 0.660 in young and old rat groups. In all rats, the correlation coefficient between the imbalance value of nNOS and Rho‐kinase and the improvement of erectile function was 0.649, P < 0.01. In conclusion, this study suggested that impaired erectile function with ageing in SD rats be associated with the imbalance between nNO and Rho‐kinase activity and Y‐27632 could improve the erectile function in old SD rats through adjusting this imbalance.