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Effects of neferine on cytosolic free calcium concentration in corpus cavernosum smooth muscle cells of rabbits
Author(s) -
Chen J.,
Liu J. H.,
Jiang Z. J.,
Wang T.,
Liu B.,
Yang J.,
Yin C. P.,
Chen F.
Publication year - 2007
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2007.00781.x
Subject(s) - phenylephrine , egta , extracellular , calcium , chemistry , endocrinology , medicine , cytosol , intracellular , smooth muscle , fura 2 , in vitro , biophysics , biology , biochemistry , enzyme , blood pressure
Summary To study the relaxation mechanisms of neferine (Nef) on the corpus cavernosum smooth muscle (CCSM), the CCSM cells from New Zealand White rabbits were cultured in vitro . [Ca 2+ ] i was measured by fluorescence ion digital imaging system (FIDIS), using Fluo‐2/AM as a Ca 2+ ‐sensitive fluorescent indicator. Nef (0.1, 1 and 10 μmol l −1 ) had no effect on the resting [Ca 2+ ] i ( P > 0.05). In the presence of extracellular Ca 2+ (2.5 mmol l −1 ), Nef (0.1, 1 and 10 μmol l −1 ) inhibited [Ca 2+ ] i elevation induced by high K + and phenylephrine (PE) in a concentration‐dependent manner ( P < 0.05). In calcium free solution containing egtaic acid (EGTA), Nef (0.1 μmol l −1 ) had no inhibitory effects on [Ca 2+ ] i elevation induced by PE ( P > 0.05). However, Nef (1 and 10 μmol l −1 ) inhibited [Ca 2+ ] i elevation induced by PE ( P < 0.05). These data suggest that Nef inhibited [Ca 2+ ] i in CCSM cells via blocking voltage‐dependent Ca 2+ channel, α 1 ‐adrenoceptor‐operated Ca 2+ channel and Ca 2+ release from intracellular Ca 2+ pool. This inhibitory action on [Ca 2+ ] i might be one of the relaxation mechanisms of Nef on the CCSM.