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Preliminary investigations on the standardisation and quality control for the determination of γ‐glutamyltranspeptidase activity in seminal plasma
Author(s) -
Chen F.,
Lu J.C.,
Xu H. R.,
Huang Y.F.,
Lu N.Q.
Publication year - 2007
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2006.00749.x
Subject(s) - centrifugation , dilution , chemistry , chromatography , semen , andrology , medicine , physics , thermodynamics
Summary The present study was designed to assess the effects of centrifugation velocity, standing time after dilution, freezing–thawing and chymotrypsin on the determination of γ ‐glutamyltranspeptidase ( γ ‐GT) activities in seminal plasma, and to establish an instruction for the standardisation and quality control for the determination of γ ‐GT within the same laboratory and among different laboratories. The γ ‐GT level and sperm concentration of each of 72 samples of seminal plasma obtained by centrifugation at 1000 g for 10 min or 3000 g for 15 min were assayed. In addition, γ ‐GT activities in diluted seminal plasma with different standing time and in samples with or without chymotrypsin were measured. The results showed that there was a significant difference of γ ‐GT levels in seminal plasma obtained by centrifugation at different velocities ( P < 0.001), and that γ ‐GT activities in seminal plasma measured after standing for 30 min after dilution were notably lower than those measured immediately after dilution ( P < 0.001). However, the data indicated that both chymotrypsin and freezing–thawing had no apparent effect on the determination of seminal γ ‐GT. In conclusion, standing time after dilution and centrifugation velocity should be standardised, and frozen seminal plasma could serve as quality control products for the determination of γ ‐GT activity among different laboratories.