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Intracytoplasmic sperm injection with motile and immotile frozen‐thawed testicular spermatozoa (the Hungarian experience)
Author(s) -
Mátyás Sz.,
Papp Gy.,
Kovács P.,
Balogh I.,
Rajczy K.,
Kopa Zs.,
Bernard A.,
Kováts T.,
Krizsa F.,
Szmatona G.,
Gáti I.,
Kaali S. G.
Publication year - 2005
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2004.00649.x
Subject(s) - intracytoplasmic sperm injection , andrology , sperm , testicular sperm extraction , biology , gynecology , medicine , embryo , microbiology and biotechnology , in vitro fertilisation
Summary The authors summarize their experience in 75 in vitro fertilization cycles, where frozen‐thawed testicular spermatozoa were used for intracytoplasmic sperm injection. In 32 cases, motile spermatozoa could be observed in the frozen‐thawed sample. In 34 cases, motility could be induced by pentoxifylline and in nine cases immotile spermatozoa, selected with hypoosmotic swelling test, were used for fertilization. The fertilization rates obtained with motile and immotile spermatozoa (66.1% versus 52.3%) were not significantly different. Our data demonstrate that freezing of testicular spermatozoa opened new possibilities for the treatment of azoospermic men. The clinical pregnancy rate per embryo transfer (ET) (21.87%) was comparable with previous results use of fresh testicular spermatozoa (27.7%). The quality and number of transferred embryos had the most significant impact on the pregnancy rate. The fertilization rate and frequency distribution of good‐quality embryos were lower in the case of immotile spermatozoa, and pregnancies were only achieved when motile spermatozoa had been used.