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Electron paramagnetic resonance spectroscopy for the investigation of the fluidity of human spermatozoa plasma membranes: a feasibility study
Author(s) -
Ochsendorf F. R.,
Rinne D.,
Fuchs J.,
Such P.,
Zimmer G.
Publication year - 2000
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.2000.tb02882.x
Subject(s) - sperm , membrane fluidity , membrane , semen , sperm motility , chemistry , electron paramagnetic resonance , andrology , biochemistry , nuclear magnetic resonance , medicine , physics
Summary. Spermatozoal membrane perturbations may play a role in abnormal sperm functions. The objective of this investigation was to study the feasibility of measuring membrane fluidity of isolated human sperm by electron paramagnetic resonance (EPR) spectroscopy and to compare the order parameter of spectra obtained from the sperm plasma membranes of living sperm of fertile men with that of infertile men. Ejaculates of infertile and fertile men were washed and the spermatozoa labelled with 5‐doxylstearic acid (5‐DSA) and 16‐doxylstearic‐acid (16‐DSA) (10 nmol per 4 times 10 7 sperm). The reporter group of 5‐DSA partitions into the outer, hydrophilic part of the sperm plasma membrane, whereas that of 16‐DSA is distributed in the inner hydrophobic part. The following results were obtained: (i) the lowest measurable cell count was 3.6 to 7 times 10 6 sperm and the interassay variance of the orderparameter s was ≤1%; (ii) swim‐up experiments revealed a higher fluidity of sperm with a higher percentage of motility; (iii) sperm membranes of infertile patients exhibited a decreased fluidity of their plasma membranes in the polar interface region of 5‐DSA compared with volunteer semen donors and fertile men ( P =0.002). No difference of membrane fluidity was found between the different groups using 16‐DSA. It is concluded that EPR spectroscopy can be used to study the fluidity of sperm plasma membranes in fertile and infertile men.