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Influence of different uropathogenic microorganisms on human sperm motility parameters in an in vitro experiment
Author(s) -
Huwe P.,
Diemer T.,
Ludwig M.,
Liu J.,
Schiefer H.G.,
Weidner W.
Publication year - 1998
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1998.tb02827.x
Subject(s) - motility , staphylococcus saprophyticus , biology , sperm motility , sperm , microbiology and biotechnology , pseudomonas aeruginosa , candida albicans , staphylococcus aureus , staphylococcus , bacteria , genetics , botany
Summary. The influence of different uropathogenic microorganisms ( E. coli, enterococcus, Pseudomonas aeruginosa, Staphylococcus saprophyticus, Candida albicans ) on human sperm motility was studied in vitro with a computer‐assisted sperm analyser (CASA). Native ejaculates were prepared with the swim‐up technique and adjusted to 22 times 10 6 spermatozoa ml −1 . The sperm suspension was artificially infected with microorganisms in concentrations varying from 2 times 10 3 to 2 times 10 7 . Sperm motility was examined directly after incubation, 2, 4 and 6 h later using the Mika motion analysis®, a computer‐based, automatic motility analysis. Former results with E. coli (serotype 06) could be confirmed that a significant inhibitory effect on sperm motility was associated with bacterial growth. Experiments with the enterococcus strain and Staphylococcus saprophyticus indicated no significant influence on sperm motility parameters. Tests with Pseudomonas aeruginosa showed a decrease of progressive motility according to time, but not to different bacterial concentrations. A significant inhibitory effect of Candida albicans was only detected in the samples with the initial bacterial concentration of 2 times 10 7 microorganisms ml −1 .