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Effect of Percoll gradient and swim‐up preparation on the chromomycin A 3 staining of normal and abnormal semen samples
Author(s) -
Georgiou I.,
Syrrou M.,
Stefanidis K.,
Konstantelli M.,
Lolis D.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1998.tb01154.x
Subject(s) - percoll , chromomycin a3 , staining , semen , andrology , biology , stain , sperm , normal group , centrifugation , medicine , endocrinology , biochemistry , karyotype , genetics , gene , chromosome
Summary The protamination of spermatozoa recovered by Percoll gradient and swim‐up was investigated by means of chromomycin A 3 (CMA 3 ) staining. A total of 34 semen samples from patients undergoing IVF treatment were divided in two groups: normal (A) and oligoasthenozoospermic (B). Samples were divided in fractions, subjected to both techniques of preparation, and stained by CMA 3 . The percentage of CMA 3 positive spermatozoa recovered by Percoll was comparable to swim‐up in the normal group. In the abnormal group Percoll resulted in significantly lower CMA 3 percentage. It is concluded that the degree of protamination in recovered spermatozoa is influenced by the technique of preparation.