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Effect of sperm motility on separation of bovine X‐ and Y‐bearing spermatozoa by means of free‐flow electrophoresis
Author(s) -
Manger M.,
Bostedt H.,
Schill W.B.,
Mileham A. J.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1997.tb03142.x
Subject(s) - sperm , electrophoresis , motility , sperm motility , andrology , free flow electrophoresis , sperm cell , gel electrophoresis , biology , chemistry , semen , chromatography , microbiology and biotechnology , genetics , biochemistry , polyacrylamide gel electrophoresis , medicine , enzyme , gel electrophoresis of proteins
Summary. Free‐flow electrophoresis was used on bovine spermatozoa to test the hypothesis that there are surface charge differences between X and Y chromosome‐bearing spermatozoa. Spermatozoa were deflected towards the anode by means of tromethamine (THAM) buffers of varying concentrations, and were separated into two populations under specific conditions. Experimental temperature and initial sperm motility had a significant effect on sperm distribution in the electric field. The results of DNA hybridization assays indicated an enrichment for Y chromosome‐bearing spermatozoa.