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Effect of fluctuations in temperature encountered during handling and shipment of human cryopreserved semen
Author(s) -
Carrell D. T.,
Wilcox A. L.,
Urry R. L.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1996.tb02808.x
Subject(s) - cryoprotectant , cryopreservation , glycerol , sperm , semen , andrology , sperm motility , yolk , liquid nitrogen , motility , chemistry , biology , food science , biochemistry , medicine , fishery , embryo , microbiology and biotechnology , organic chemistry
Summary. The effect of temperature fluctuations which cryopreserved spermatozoa may undergo during routine shipping and handling was evaluated in sperm frozen with two cryoprotectants. Sperm frozen in TEST‐yolk buffer maintained motility better than those frozen in glycerol solution in all studies. Sperm motility was significantly compromised in samples stored more than one day in dry ice, regardless of the cryoprotectant, and more than two days in a liquid nitrogen shipping dewar if frozen in glycerol solution. Sperm motility was not compromised following exposure to room temperature for up to 3 min if TEST‐yolk buffer was used as the cryoprotectant, but was compromised following 1 min exposure using glycerol cryoprotectant. This study describes the limits of non‐ideal conditions that spermatozoa may undergo during shipping and handling, and demonstrates the effects of the cryoprotectant on those limits.