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Use of computerized motility analyzer for the evaluation of frozen‐thawed ram spermatozoa
Author(s) -
Moses D. F.,
Heras M. A.,
Valcárcel A.,
Pérez L.,
Baldassarre H.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1995.tb02091.x
Subject(s) - semen , motility , curvilinear coordinates , andrology , insemination , spectrum analyzer , chemistry , materials science , anatomy , biomedical engineering , biology , sperm , physics , medicine , mathematics , optics , geometry , microbiology and biotechnology
Summary A computerized motility analyzer (CellTrak/S TM ) was calibrated for its use with ram semen. Adjustment of the several setup variables allowed an accurate measurement of kinetic parameters such as percentage of motile cells, straight velocity, curvilinear velocity, linearity and amplitude of lateral displacement of the head. All kinetic parameters, except the lateral displacement of the head, showed significant changes after freezing and thawing. The curvilinear velocity exhibited the least significative post‐thaw decrease. The alterations in kinetic parameters provoked by freezing and thawing could account for the low success obtained with frozen semen by cervical insemination, as it is accepted that during the initial steps of fertilization high motility and linearity are required.