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Effect of lipid peroxidation on beating frequency of human sperm tail
Author(s) -
Hong C. Y.,
Lee M. F.,
Lai L.J.,
Wang C. P.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1994.tb00757.x
Subject(s) - lipid peroxidation , sperm , superoxide dismutase , catalase , sperm motility , chemistry , antioxidant , glutathione , andrology , biochemistry , enzyme , medicine
Summary. The tail beat frequency (TBF) of sperm was measured with a sperm‐head fixation method which was based on the tendency of sperm to attach its head to the surface of a glass slide. Ferrous sulphate, a promotor of lipid peroxidation, inhibited TBF within 5 min of mixing with sperm. This inhibition can be reversed dose‐dependently by superoxide dismutase, catalase, glutathione, and albumin. It was concluded that TBF could be a new pharmacological model for studying the effect of lipid peroxidation and antioxidant on sperm motility. Both enzymatic and non‐enzymatic antioxidants can be screened with this method.