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Enhanced quality of capacitated spermatozoa from oligoasthenozoospermic men after incubation in test yolk medium
Author(s) -
Ragni G.,
Finzi G. C. Lombroso,
Caccamo A.,
Serra A. Dalla,
Crosignani P. G.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1993.tb02738.x
Subject(s) - incubation , andrology , yolk , sperm , sperm quality , semen , biology , male infertility , semen analysis , infertility , medicine , pregnancy , genetics , biochemistry , ecology
Summary. To improve artificially the quality of poor sperm samples to be used in assisted procreation procedures, Test Yolk Buffer (TYB) incubation effect was investigated. After adding the TYB procedure to the standard swim‐up technique, a significant ( P < 0.001) increase in spermatozoa velocity was observed, while the other seminal parameters were not affected. Three of 11 couples who had not had success after three intraperitoneal inseminations (IPI) with sperm prepared by the usual swim‐up technique obtained pregnancies after IPI with TYB‐incubated spermatozoa. These preliminary results suggest that TYB incubation for capacitated spermatozoa be employed in the assisted procreation strategies applied for male infertility.