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Expression of the proliferation‐associated Ki‐67 antigen in bovine testicular tubular cells during the seminiferous epithelial cycle, demonstrated with the MIB‐1 antibody
Author(s) -
Wrobel K.H.,
Kujat R.,
Lutz R.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1993.tb02729.x
Subject(s) - seminiferous tubule , sertoli cell , meiosis , prophase , biology , immunohistochemistry , andrology , spermatogenesis , anaphase , antigen , antibody , monoclonal antibody , microbiology and biotechnology , apoptosis , cell cycle , endocrinology , immunology , genetics , gene , medicine
Summary. Ki‐67 expression in the seminiferous tubule of the bovine testis was studied by immunohistochemistry during the seminiferous epithelial cycle using the monoclonal antibody MIB‐1. Spermatogonial proliferation is most obvious in stages 5–7, and 8, when B‐spermatogonia divide. A lower rate of spermatogonial propagation is observed preceding or during meiosis in stages 1–4. The MIB‐1 antibody also gives positive results with some post‐spermatogonial tubular cells. Preleptotenes passing through S‐phase in stage 1 reveal positive nuclei. During prophase of meiosis I pachytenes react strongly, diplotenes react in an attenuated manner, while leptotenes and zygotenes stay negative. Secondary spermatocytes seen in stage 4 are positive as are the chromosomes during meta‐ and anaphase of the meiotic divisions. Post‐meiotic spermatids are also decorated but stop Ki‐67 expression abruptly at the end of stage 4. Sertoli cells are negative.