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Maturation, capacitation, and metabolism of goat spermatozoa using magnetic resonance methods
Author(s) -
Srivastava S.,
Kaur R.,
Bawa S. R.,
Phadke R. S.,
Bains H. K.,
Sehgal S.,
Govil G.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1993.tb02713.x
Subject(s) - capacitation , in vitro , sperm , chemistry , kinetics , andrology , metabolism , nuclear magnetic resonance , biochemistry , microbiology and biotechnology , biology , medicine , physics , quantum mechanics
Summary. The ability of intact cells to reduce spin labels has been utilized to characterize the activity of spermatozoa of goat. The kinetics of reduction of TEMPO has been found to be sensitive to the quantity, quality and state of epididymal maturation of the spermatozoa. Presence of alcohol caused activation and Gossypol acetic acid left sperm activity unaltered. Electron spin resonance (ESR) and 31 P Nuclear magnetic resonance (NMR) indicate that the period of in vitro capacitation requires optimization. 31 P NMR spectra indicate a good correlation with the progressive maturation of the cells.