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Effect of calmodulin‐like protein from buffalo ( Bubalus bubalis ) seminal plasma on Ca 2+ , Mg 2+ ‐ATPase of purified plasma membrane of buffalo spermatozoa
Author(s) -
Sidhu K. S.,
Guraya S. S.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1993.tb02677.x
Subject(s) - bubalus , calmodulin , atpase , semen , plasma membrane ca2+ atpase , biology , chemistry , andrology , microbiology and biotechnology , biochemistry , enzyme , anatomy , medicine , ecology
Summary. Buffalo sperm heads and tails were cleaved by sonication and isolated in relatively pure proportions i.e. 95% and 98% respectively, by discontinuous sucrose density‐gradient centri‐fugation. Purified plasma membranes from the isolated sperm heads and tails were obtained by hypotonic treatment and brief sonication followed by discontinuous sucrose density‐gradient centrifugation. Ca 2+ , Mg 2+ ‐ATPase activity was evident in plasma membrane from sperm heads and tails, although activity was greater in the latter. A calmodulin‐like protein isolated from buffalo seminal plasma increased the Ca 2+ , Mg 2+ ‐ATPase of plasma membrane from the sperm heads and tails by 128 and 136% respectively. Based upon the data obtained here and elsewhere (Sidhu & Guraya, 1989a) a model is proposed which explains regulation of Ca 2+ in buffalo spermatozoa and implicates calmodulin‐like protein and Ca 2+ , Mg 2+ ‐ATPase in sperm acrosome reaction.