Premium
Y‐chromosome‐specific fluorescence (f‐body) of poorly decondensed bovine spermatozoa
Author(s) -
Blottner S.,
Pitra C.,
Berger U.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1992.tb02650.x
Subject(s) - fluorescent staining , boar , staining , stain , fluorescence , biology , papain , andrology , microbiology and biotechnology , y chromosome , body weight , chemistry , semen , anatomy , genetics , biochemistry , gene , endocrinology , medicine , physics , quantum mechanics , enzyme
Summary. An account is given in this paper of a method of identifying the fluorescence body (f‐body) as a marker of the Y‐chromosome. Also covered by this method is poor decondensation of spermatozoal nuclei when exposed to action of 1.25% of papain, 0.155% of DTE, and 0.025% of DMSO. Quinacrine mustard was used as fluorescent stain, its final concentration being 0.0025%. For staining, spermatozoa were suspended for 1–5 h. Average f‐body frequency accounted for 41.0 ± 5.1% in 35 ejaculates from 22 bulls. The overall variation coefficient amounted to 12.4% and thus was higher than each of the single values individually recorded from six bulls which were involved with three or four ejaculates (3.6–7.0%). F‐bodies could not be detected by the method generally used on human spermatozoa. The applicability of the f‐body test to quantification of Y spermatozoa in experimental separation of androspermatozoa and gynospermatozoa is discussed and is demonstrated by an example.